《植物生理学报》 2016, 52(2): 167-176

胞外多肽激素基因CHAL/CLL1/CLL2在拟南芥雄蕊发育过程中发挥重要作用

何卓娜^1,2, 王双双^1,2, 马红^1,2, 常芳^1,2,*

复旦大学¹生命科学学院, ²植物科学研究所, 上海200438

通信作者：常芳；E-mail: fangchang@fudan.edu.cn

摘　要：

细胞-细胞间通讯是雄蕊正常发育过程所必需的, 然而目前对于参与此过程的信号分子却鲜有报道。前期报道CHAL/CLL1/CLL2胞外多肽激素在拟南芥气孔发育过程中发挥重要作用, 但是chal cll1 cll2三突变体同时表现出植株育性异常的表型。然而, 这三个基因在植物雄蕊发育过程中的表达和具体功能并未见深入研究。本研究发现CHAL/CLL1/CLL2这三个基因在拟南芥花丝及5-9期花药的绒毡层和生殖细胞中高表达。同时, 对三个基因功能缺失突变体的雄性育性情况进行分析后发现: 单基因突变植株未表现出明显的育性异常, 但双突(chal cll1和chal cll2)和三突(chal cll1 cll2)植株育性逐级下降, 尤其是三突表现出一系列雄蕊异常的表型: 花丝短小、花药绒毡层发育异常、部分花粉败育, 且花粉萌发率亦较野生型有显著降低。进一步研究发现, chal cll1 cll2三突变体中雄蕊发育途径中关键基因BAM1、EMS1、TPD1、bHLH089、TSM1、MS1和AMS的表达水平发生显著变化。以上结果一致表明CHAL/CLL1/CLL2基因在拟南芥雄蕊发育过程中发挥重要作用。

关键词：拟南芥; 花药; 胞外多肽激素; CHAL、CLL1、CLL2。

收稿：2015-12-16   修定：2016-01-19

资助：国家自然科学基金委重点项目(31130006)。 致谢 感谢Salk研究所基因组研究实验室(Salk Institute Genomic Analysis Laboratory)以及俄亥俄州立大学拟南芥生物资源中心(Ohio State University Arabidopsis Biological Center)为本研究提供chal、chal cll1、chal cll2、以及chal cll1 cll2突变体种子。

Peptide hormones CHAL, CLL1, and CLL2 are important for stamen development in Arabidopsis

HE Zhuo-Na^1,2, WANG Shuang-Shuang^1,2, MA Hong^1,2, CHANG Fang^1,2,*

¹School of Life Sciences, ²Institute of Plant Biology, Fudan University, Shanghai 200438, China

Corresponding author: CHANG Fang; E-mail: fangchang@fudan.edu.cn

Abstract:

Cell-cell communication is important for normal anther development and male fertility, but little is known about signals important for this process. The polypeptide hormones CHAL/CLL1/CLL2 are redundantly required for normal stomata cell development in Arabidopsis, but the chal cll1 cll2 triple mutants exhibited reduced fertility. However, the function of the CHAL/CLL1/CLL2 genes in the regulation of Arabidopsis fertility has not been investigated. In this study, we showed that the CHAL/CLL1/CLL2 genes were highly expressed in anther filaments, tapetal cells, and male reproductive cells at anther stage 5–9. Single mutants of the CHAL/CLL1/CLL2 genes did not show obvious fertility defects, but the double (chal cll1 and chal cll2) and triple mutants (chal cll1 cll2) showed incremental defects in fertility: short filaments, abnormal tapetal development, aborted pollens, and extremely low pollen germination rate. Further studies showed that, the expression of many anther development-required genes were obviously altered in the chal cll1 cll2 triple mutant, including BAM1, EMS1, TPD1, bHLH089, TSM1, MS1, and AMS. These data together strongly support the idea that CHAL/CLL1/CLL2 play important roles in Arabidopsis stamen development.

Key words: Arabidopsis thaliana; anther; polypeptide hormone; CHAL, CLL1, CLL2